Journal:

Article Title: Expression of the Two Major Envelope Proteins of Equine Arteritis Virus as a Heterodimer Is Necessary for Induction of Neutralizing Antibodies in Mice Immunized with Recombinant Venezuelan Equine Encephalitis Virus Replicon Particles

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Figure Lengend Snippet: Induction of neutralizing antibodies in mice with EAV-VRPs and whole EAV

Article Snippet: The neutralizing-antibody titers in these mice were comparable to or higher than those in mice repeatedly immunized with whole EAV (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Immunogen Immunization schedule (weeks) Dose (IU a or TCID 50 b ) Weeks after primary immunization Neutralizing antibody titers c of mouse no.: 1 2 3 4 pVR21-G L VRP Primary (0) 6 × 10 5 3 0 0 0 0 First boost (3) 5 0 0 0 0 Second boost (7) 9 0 0 0 0 pVR21-M VRP Primary (0) 6 × 10 6 3 0 0 0 0 First boost (3) 5 0 0 0 0 Second boost (7) 9 0 0 0 0 pVR100-G L /M VRP Primary (0) 3.6 × 10 6 3 32 32 64 64 First boost (3) 5 512 512 512 512 Second boost (7) 9 2,048 1,024 2,048 2,048 EAV ATCC strain Primary (0) 2 × 10 4 b N/A d ND e ND ND ND First boost (4) N/A ND ND ND ND Second boost (7) 9 1,024 1,024 1,024 512 Open in a separate window a Infectious units as determined by immunoperoxidase staining of infected BHK-21 cells. b 50% tissue culture infectious doses. c Neutralization titers are expressed as the inverse of the antibody dilution providing 50% protection of RK-13 cell monolayers against 200 50% tissue culture infective doses of virus. d N/A, not applicable. e ND, not determined.

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